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CyFlow™ CD79a PE

CyFlow™ CD79a PE
Application: Flow cytometry
Target Species: Chicken, Cow, Dog, Guinea pig, Horse, Human, Mouse, Non-Human Primates, Pig, Rabbit, Rat
Alternative Name: IgA, MB-1, MB1
Antigen: CD79a
Clonality: monoclonal
Isotype: IgG1
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Laser: Blue, Green, Yellow
Format/Fluorochrome: PE
Field of Interest: Immunophenotyping
Source Species: Mouse
Regulatory Status: RUO
Clone: HM47
Order number: CU479884
Quantity 100 tests Volume 1.0 mL Immunogen Synthetic peptide corresponding to C... more
Product information "CyFlow™ CD79a PE"
Quantity100 tests
Volume1.0 mL
ImmunogenSynthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
Background InformationCD79a (Ig α, MB1) forms disulfide-linked heterodimer with CD79b (Ig β, B29). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with λ5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Technical Data Sheet

Instruction for Use

ECDoC

Certificate Of Analysis

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