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CyFlow™ CD19 Pacific Blue™

CyFlow™ CD19 Pacific Blue™
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Excitation Maximum: 400 to 410 nm
Emission Maximum: 455 nm
Antigen: CD19
Alternative Name: B4
Clone: LT19
Order number: BN140719
HLDA Workshop HCDM/HLDA 10 Quantity 100 tests Volume 0.4 mL Immunogen Daudi human... more
Product information "CyFlow™ CD19 Pacific Blue™"
HLDA WorkshopHCDM/HLDA 10
Quantity100 tests
Volume0.4 mL
ImmunogenDaudi human Burkitt lymphoma cell line
Background InformationCD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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