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CyFlow™ CD71 Biotin
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Target Species: | Human |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Field of Interest: | Endocytosis, Immunophenotyping |
| Isotype: | IgG1 |
| Format/Fluorochrome: | Biotin |
| Antigen: | CD71 |
| Alternative Name: | T9, TFR, TRFR |
| Clone: | MEM-75 |
| Order number: | CF637400 |
For Research Use Only
Not for use in diagnostic or therapeutic procedures.
HLDA Workshop HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 Concentration Unit mg/mL... more
Product information "CyFlow™ CD71 Biotin"
| HLDA Workshop | HLDA IV—WS Code A 045 HLDA V—WS Code T T-165 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | NALM-6 human pre-B cell line |
| Background Information | CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
Technical Data Sheet
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