Welcome to
Sysmex Flow Cytometry France!

For ordering please make sure you are in your local shop.
If you are from France then please continue.
Otherwise please go to your country.

Go to my country Continue

Article successfully added.

CyFlow™ CD64 Biotin

CyFlow™ CD64 Biotin
Antigen: CD64
Alternative Name: FcγRI
Clone: 10.1
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: AM698743

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA III—WS Code M-250 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD64 Biotin
HLDA WorkshopHLDA III—WS Code M-250
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenRheumatoid synovial fluid cells and fibronectin purified human monocytes
Background InformationCD64 (FcγRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding α subunit and promiscuous γ subunit, which is indispensable for tyrosine-based signaling. However, even the α subunit can transduce signals leading to cellular effector functions. The isoform FcγRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcγRIb and FcγRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Dougherty GJ, Selvendran Y, Murdoch S, Palmer DG, Hogg N: The human mononuclear phagocyte high‑affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1. Eur·J·Immunol. 1987·Oct; 17(10):1453‑9. <·PMID:·3500057·> | Jayaram Y, Buckle AM, Hogg N: The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon‑gamma. Clin·Exp·Immunol. 1989·Mar; 75(3):414‑20. <·PMID:·2495203·> | Hashimoto S, Yamada M, Motoyoshi K, Akagawa KS: Enhancement of macrophage colony‑stimulating factor‑induced growth and differentiation of human monocytes by interleukin‑10. Blood. 1997·Jan·1; 89(1):315‑21. <·PMID:·8978307·> | Fadlon E, Vordermeier S, Pearson TC, Mire-Sluis AR, Dumonde DC, Phillips J, Fishlock K, Brown KA: Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64. Blood. 1998·Jan·1; 91(1):266‑74. <·PMID:·9414294·> | Brichard B, Varis I, Latinne D, Deneys V, de Bruyere M, Leveugle P, Cornu G: Intracellular cytokine profile of cord and adult blood monocytes. Bone·Marrow·Transplant. 2001·May; 27(10):1081‑6. <·PMID:·11438825·> | Sánchez-Torres C, García-Romo GS, Cornejo-Cortés MA, Rivas-Carvalho A, Sánchez-Schmitz G: CD16+ and CD16‑ human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int·Immunol. 2001·Dec; 13(12):1571‑81. <·PMID:·11717198·> | Beekman JM, Bakema JE, van der Linden J, Tops B, Hinten M, van Vugt M, van de Winkel JG, Leusen JH: Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin. J·Biol·Chem. 2004·Aug·6; 279(32):33875‑81. <·PMID:·15161926·> | Devaraj S, Du Clos TW, Jialal I: Binding and internalization of C‑reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects. Arterioscler·Thromb·Vasc·Biol. 2005·Jul; 25(7):1359‑63. <·PMID:·15860734·> | Roura-Mir C, Wang L, Cheng TY, Matsunaga I, Dascher CC, Peng SL, Fenton MJ, Kirschning C, Moody DB: Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR‑2. J·Immunol. 2005·Aug·1; 175(3):1758‑66. <·PMID:·16034117·> | Devaraj S, Davis B, Simon SI, Jialal I: CRP promotes monocyte‑endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions. Am·J·Physiol·Heart·Circ·Physiol. 2006·Sep; 291(3):H1170‑6. <·PMID:·16603696·>