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CyFlow™ CD63 Biotin

CyFlow™ CD63 Biotin
Antigen: CD63
Alternative Name: LAMP-3, LIMP, MLA1
Clone: MEM-259
Application: Flow cytometry
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Endocytosis, Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: CL410266

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 Immunogen... more
CyFlow™ CD63 Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD63 (LAMP-3; lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to theendosomal‑lysosomal‑MIICs route after extracellular stimuli in human immature dendritic cells. Blood. 2004·Aug·15; 104(4):1183‑90. <·PMID:·15130945·> | Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T: The small chemical vacuolin‑1 inhibits Ca(2+)‑dependent lysosomal exocytosis but not cell resealing. EMBO·Rep. 2004·Sep; 5(9):883‑8. <·PMID:·15332114·> | Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP‑1 and LAMP‑2, but not LAMP‑3, are reliable markers for activation‑induced secretion of human mast cells. Cytometry·A. 2004·Sep; 61(1):62‑8. <·PMID:·15351990·> | Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W: CD63 as an activation‑linked T cell costimulatory element. J·Immunol. 2004·Nov·15; 173(10):6000‑8. <·PMID:·15528334·> | Israels SJ, McMillan-Ward EM: CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb·Haemost. 2005·Feb; 93(2):311‑8. <·PMID:·15711748·> | Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ: CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung. Lung·Cancer. 2007·Jul; 57(1):46‑53. <·PMID:·17350713·> | Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH: Expression of tetraspan protein CD63 activates protein tyrosine kinase (PTK) and enhances the PTK‑induced inhibition of ROMK channels. J·Biol·Chem. 2008·Jan·22; <·PMID:·19880522·>