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CyFlow™ CD63 Alexa Fluor™ 488

CyFlow™ CD63 Alexa Fluor™ 488
Application: Flow cytometry
Excitation Maximum: 495 nm
Clone: MEM-259
Alternative Name: LAMP-3, LIMP, MLA1
Antigen: CD63
Emission Maximum: 519 nm
Format/Fluorochrome: Alexa Fluor™ 488
Clonality: monoclonal
Isotype: IgG1
Field of Interest: Endocytosis, Immunophenotyping
Source Species: Mouse
Laser: Blue
Regulatory Status: RUO
Target Species: Human
Order number: CE360165
Quantity 100 tests Volume 0.4 mL Immunogen Human Acute Lymphocytic Leukemia (ALL) T... more
Product information "CyFlow™ CD63 Alexa Fluor™ 488"
Quantity100 tests
Volume0.4 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Background InformationCD63 (LAMP-3; lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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