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CyFlow™ CD62L Alexa Fluor™ 700

CyFlow™ CD62L Alexa Fluor™ 700
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Laser: Red
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 700
Excitation Maximum: 702 nm
Emission Maximum: 723 nm
Antigen: CD62L
Alternative Name: L-selectin
Clone: DREG56
Order number: AU807163
Quantity 100 tests Volume 0.4 mL Immunogen PMA-activated human peripheral blood... more
Product information "CyFlow™ CD62L Alexa Fluor™ 700"
Quantity100 tests
Volume0.4 mL
ImmunogenPMA-activated human peripheral blood leukocytes
Background InformationCD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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