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CyFlow™ CD55 PE-Cy5

CyFlow™ CD55 PE-Cy5
Antigen: CD55
Alternative Name: DAF
Clone: MEM-118
Application: Flow cytometry
Format/Fluorochrome: PE-Cy5
Laser: Blue, Green, Yellow
Target Species: Human, Non-Human Primates
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 670 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgM
Order number: CK237219

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen HPB-ALL human T cell line Specifity... more
CyFlow™ CD55 PE-Cy5
Quantity100 tests
Volume0.4 ml
ImmunogenHPB-ALL human T cell line
SpecifityThe mouse monoclonal antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 antigen, a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.
Background InformationCD55 (DAF; decay-accelerating factor) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can still regulate complement when bound to CD97. In tumors, besides protection agains complement, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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