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CyFlow™ CD53 Low Endotoxin

CyFlow™ CD53 Low Endotoxin
Antigen: CD53
Alternative Name: TSPAN2
Clone: MEM-53
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot
Format/Fluorochrome: Low Endotoxin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BY891522

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Concentration 1 mg/ml Quantity 0.1 mg Volume 0.1 ml Immunogen Leukocytes of a... more
CyFlow™ CD53 Low Endotoxin
Concentration1 mg/ml
Quantity0.1 mg
Volume0.1 ml
ImmunogenLeukocytes of a patient suffering from LGL-type Leukemia
SpecifityThe mouse monoclonal antibody MEM-53 recognizes CD53 antigen, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Background InformationCD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumor cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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