Welcome to
Sysmex Flow Cytometry France!

For ordering please make sure you are in your local shop.
If you are from France then please continue.
Otherwise please go to your country.

Go to my country Continue

CyFlow™ CD50 Alexa Fluor™ 488

CyFlow™ CD50 Alexa Fluor™ 488
Antigen: CD50
Alternative Name: ICAM-3
Clone: MEM-171
Application: Flow cytometry
Format/Fluorochrome: Alexa Fluor™ 488
Laser: Blue
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Isotype: IgG1
Order number: AD551837

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 ml Immunogen Human granulocytes Specifity The mouse... more
CyFlow™ CD50 Alexa Fluor™ 488
Quantity100 tests
Volume0.4 ml
ImmunogenHuman granulocytes
SpecifityThe mouse monoclonal antibody MEM-171 recognizes an epitope in the D2 domain of CD50 antigen, a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
Background InformationCD50 ( ICAM-3; intracellular adhesion molecule 3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (DC-SIGN; dendritic cell-specific ICAM-3-grabbing nonintegrin), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Article successfully added.