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CyFlow™ CD45R0 FITC

CyFlow™ CD45R0 FITC
Antigen: CD45R0
Alternative Name: UCHL-1
Clone: UCHL1
Application: Flow cytometry
Format/Fluorochrome: FITC
Laser: Blue
Target Species: Human
Field of Interest: Immunophenotyping, Phosphorylation
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Isotype: IgG2a
Order number: BA212505

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code NL 31 HLDA V—WS... more
CyFlow™ CD45R0 FITC
HLDA WorkshopHLDA III—WS Code NL 826 HLDA III—WS Code T 128 HLDA IV—WS Code NL 31 HLDA V—WS Code BP BP460 HLDA V—WS Code T T-081 HLDA V—WS Code T T-CD45.43
Quantity100 tests
Volume2
ImmunogenHuman IL-2 dependent T cells
Background InformationCD45R0 (UCHL-1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45R0 is expressed e.g. on macrophages, CD8+ T cells, activated T cells and myeloma cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG: Monoclonal antibody (UCHL{01} that recognises normal and neoplastic T cells in routinely fixed tissues. J·Clin·Pathol. 1986·Apr; 39(4):399‐405. <·PMID:·2422219·> | Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC: Functional subsets of human helper‑inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986·May; 58(1):63‑70. <·PMID:·2423439·> | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Beverley PC: Human T cell subsets. Immunol·Lett. 1987·Apr; 14(4):263‑7. <·PMID:·2953677·> | Beverley PC, Merkenschlager M, Terry L: Phenotypic diversity of the CD45 antigen and its relationship to function. Immunol·Suppl. 1988; 1:3‑5. <·PMID:·2968306·> | Akbar AN, Terry L, Timms A, Beverley PC, Janossy G: Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J·Immunol. 1988·Apr·1; 140(7):2171‑8. <·PMID:·2965180·> | Terry LA, Brown MH, Beverley PC: The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte‑common antigen, CD45. Immunology. 1988·Jun; 64(2):331‑6. <·PMID:·2455685·> | Merkenschlager M, Terry L, Edwards R, Beverley PC: Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur·J·Immunol. 1988·Nov; 18(11):1653‑61. <·PMID:·2974420·> | Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·> | Li FJ, Tsuyama N, Ishikawa H, Obata M, Abroun S, Liu S, Otsuyama K, Zheng X, Ma Z, Maki Y, Kawano MM: A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL‑6‑induced proliferation in myeloma. Blood. 2005·Apr·15; 105(8):3295‑302. <·PMID:·15626731·> | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·> | Cosenza-Nashat MA, Kim MO, Zhao ML, Suh HS, Lee SC: CD45 isoform expression in microglia and inflammatory cells in HIV‑1 encephalitis.. Brain·Pathol. 2006·Oct; 16(4):256‑65. <·PMID:·17107594·> | Bijian K, Zhang L, Shen SH: Collagen‑mediated survival signaling is modulated by CD45 in Jurkat T cells. Mol·Immunol. 2007·Jul; 44(15):3682‑90. <·PMID:·17524482·> | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·>