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CyFlow™ CD36 PE

CyFlow™ CD36 PE
Antigen: CD36
Alternative Name: GP3B, GPIV
Clone: CB38 (NL07)
Application: Flow cytometry
Format/Fluorochrome: PE
Laser: Blue, Green, Yellow
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Isotype: IgM
Order number: BZ332383

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA IV—WS Code P106 Quantity 100 tests Volume 1 Immunogen Living... more
CyFlow™ CD36 PE
HLDA WorkshopHLDA IV—WS Code P106
Quantity100 tests
Volume1
ImmunogenLiving human myeloid cells
Background InformationCD36 (FAT; fatty acid translocase) is an 85-113 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing Tris buffered saline (TBS) solution, pH ≈8.0, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·> | Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr·Stud·Hematol·Blood·Transfus. 1991; 58:182‑6. <·PMID:·1720081·> | Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F: Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell·Immunol. 1991·Oct·15; 137(2):487‑500. <·PMID:·1716523·> | Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F: Platelet activation and inhibition of malarial cytoadherence by the anti‑CD36 IgM monoclonal antibody NL07. Blood. 1993·Dec·15; 82(12):3637‑47. <·PMID:·7505121·> | Fadok VA, Warner ML, Bratton DL, Henson PM: CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J·Immunol. 1998·Dec·1; 161(11):6250‑7. <·PMID:·9834113·> | Matasić R, Dietz AB, Vuk-Pavlović S: Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells. J·Leukoc·Biol. 1999·Dec; 66(6):909‑14. <·PMID:·10614771·> | Kapinsky M, Torzewski M, Büchler C, Duong CQ, Rothe G, Schmitz G: Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger‑receptor CD36. Arterioscler·Thromb·Vasc·Biol. 2001·Jun; 21(6):1004‑10. <·PMID:·11397711·> | McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH: Immunophenotypic analysis of hematogones (B‑lymphocyte precursors) in 662 consecutive bone marrow specimens by 4‑color flow cytometry. Blood. 2001·Oct·15; 98(8):2498‑507. <·PMID:·11588048·> | Hoffmann PR, deCathelineau AM, Ogden CA, Leverrier Y, Bratton DL, Daleke DL, Ridley AJ, Fadok VA, Henson PM: Phosphatidylserine (PS) induces PS receptor‑mediated macropinocytosis and promotes clearance of apoptotic cells. J·Cell·Biol. 2001·Nov·12; 155(4):649‑59. <·PMID:·11706053·> | Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin·Vaccine·Immunol. 2006·Jun; 13(6):704‑7. <·PMID:·16760331·>