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CyFlow™ CD31 Alexa Fluor™ 488

CyFlow™ CD31 Alexa Fluor™ 488
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue
Source Species: Mouse
Field of Interest: Immunophenotyping
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 488
Excitation Maximum: 495 nm
Emission Maximum: 519 nm
Antigen: CD31
Alternative Name: endoCAM, PECA1, PECAM-1
Clone: MEM-05
Order number: AL676342
Quantity 100 tests Volume 0.4 mL Immunogen Leukocytes of a patient suffering from... more
Product information "CyFlow™ CD31 Alexa Fluor™ 488"
Quantity100 tests
Volume0.4 mL
ImmunogenLeukocytes of a patient suffering from LGL-type leukemia
Background InformationCD31 (PECAM-1; platelet endothelial cell adhesion molecule-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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