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CyFlow™ CD4 Alexa Fluor™ 700

CyFlow™ CD4 Alexa Fluor™ 700
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Field of Interest: Immunophenotyping
Laser: Red
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 700
Excitation Maximum: 702 nm
Emission Maximum: 723 nm
Antigen: CD4
Alternative Name: Leu3a, T4
Clone: MEM-241
Order number: CM002106
HLDA Workshop HCDM (HLDA VIII)—WS Code M241 Quantity 100 tests Volume 0.4 mL... more
Product information "CyFlow™ CD4 Alexa Fluor™ 700"
HLDA WorkshopHCDM (HLDA VIII)—WS Code M241
Quantity100 tests
Volume0.4 mL
Immunogen2 N-terminal domains of human CD4 fused to human IgG1 Fc
Background InformationCD4 (T4, Leu3a) is a 55 kDa single-chain transmembrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1, 2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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